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M9480863.TXT
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1994-09-05
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Document 0863
DOCN M9480863
TI Identification of HIV-1 variants transmitted from mothers to fetus.
DT 9410
AU Furtado MR; Hoeft J; Garcia PM; Department of Pathology, Northwestern
University, Chicago, IL; 60611.
SO Abstr Gen Meet Am Soc Microbiol. 1994;94:482 (abstract no. T-2). Unique
Identifier : AIDSLINE ASM94/94313082
AB Identification of HIV-1 sequence variants that are transmitted to
infants and determination of the timing of transmission are crucial to
understanding the underlying mechanisms. We analyzed fetal tissue from
five abortuses and detected HIV-1 DNA in four. Analysis for maternal DNA
in the fetal samples by PCR amplification of the apo-B VNTR locus
detected contaminating DNA in one of the samples. The C2V3 to V4V5
region of HIV-1 envelope gene was amplified from three maternal PBMC
samples and corresponding fetal tissue, inserted into plasmid vectors
and sequenced. Phylogenetic analysis of the sequence variants indicated
that viral sequences found in fetal tissue exhibited a low degree of
divergence and were highly homologous to some of the variants in the
mother. Our studies suggest that transmission could occur early in
gestation.
DE Abortion Acquired Immunodeficiency Syndrome/*TRANSMISSION Female
Fetus/*MICROBIOLOGY Human HIV-1/*GENETICS/ISOLATION & PURIF
Polymerase Chain Reaction Pregnancy Pregnancy Complications,
Infectious/*MICROBIOLOGY Support, Non-U.S. Gov't *Variation (Genetics)
Viral Envelope Proteins/GENETICS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).